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In the present article, we will discuss the RNA sequencing only but before that read our previous article on transcriptomics: What Is Transcriptomics?

Here in the RNA sequencing, the cDNA synthesised from the mRNA is sequenced and quantified in a sequencer. Lifestyle, diet, exercise, environment, conditions and other external factors play a significant role in transcriptome regulation. The transcriptome varies in different condition and a different environment. The transcriptome of an organism is larger, complex and more uncertain, unlike the genome. One of the important techniques used in transcriptomics studies is RNA sequencing. Transcriptomics is the study of the entire mRNA- often called as transcriptomes or total RNA present in a cell or cell population.īy analysing the transcriptome of a cell, the entire pool of the gene expression can be examined. Means, the amount of total RNA, mRNA and ncRNA can be determined by studying the total RNA. Henceforth, by analysing an mRNA, the expression pattern of the related gene can be determined.Īlso, total RNA examination gives us an idea about which genes are linked to the protein formation and which are not. Thus it only possesses the sequences which are required in the protein formation. The mRNA or transcript contains only the coding sequences from a gene.